singleplex real-time pcr  (Thermo Fisher)

Journal: Diagnostics

Article Title: Evaluation of the Use of Singleplex and Duplex CerTest VIASURE Real-Time PCR Assays to Detect Common Intestinal Protist Parasites

doi: 10.3390/diagnostics14030319

Figure Lengend Snippet: Main features of the CerTest VIASURE Real-Time PCR detection kits.

Article Snippet: Five singleplex CerTest VIASURE Real-Time PCR kits used to detect Cryptosporidium spp., D. fragilis , E. dispar , E. histolytica , and G. duodenalis were evaluated, and a duplex CerTest VIASURE Real-Time PCR kit used to simultaneously detect Blastocystis sp. and D. fragilis was also included in the study.

Techniques: Real-time Polymerase Chain Reaction

Journal: Diagnostics

Article Title: Evaluation of the Use of Singleplex and Duplex CerTest VIASURE Real-Time PCR Assays to Detect Common Intestinal Protist Parasites

doi: 10.3390/diagnostics14030319

Figure Lengend Snippet: Direct comparison of the CerTest VIASURE Real-Time PCR detection kits to reference PCR methods used during routine analyses in the initial diagnosis.

Article Snippet: Five singleplex CerTest VIASURE Real-Time PCR kits used to detect Cryptosporidium spp., D. fragilis , E. dispar , E. histolytica , and G. duodenalis were evaluated, and a duplex CerTest VIASURE Real-Time PCR kit used to simultaneously detect Blastocystis sp. and D. fragilis was also included in the study.

Techniques: Comparison, Real-time Polymerase Chain Reaction

Journal: Diagnostics

Article Title: Evaluation of the Use of Singleplex and Duplex CerTest VIASURE Real-Time PCR Assays to Detect Common Intestinal Protist Parasites

doi: 10.3390/diagnostics14030319

Figure Lengend Snippet: Diagnostic performance of the VIASURE Real-Time PCR detection kits when applied to PCR-confirmed samples during routine analyses in initial diagnosis.

Article Snippet: Five singleplex CerTest VIASURE Real-Time PCR kits used to detect Cryptosporidium spp., D. fragilis , E. dispar , E. histolytica , and G. duodenalis were evaluated, and a duplex CerTest VIASURE Real-Time PCR kit used to simultaneously detect Blastocystis sp. and D. fragilis was also included in the study.

Techniques: Diagnostic Assay, Real-time Polymerase Chain Reaction

Journal: Virulence

Article Title: Development of a Taq Man-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses

doi: 10.1080/21505594.2020.1771980

Figure Lengend Snippet: Cq values of PEDV, PDCoV, PToV, and SADS-CoV detected by this multiplex real-time PCR assay with different probe and primer concentrations.

Article Snippet: As shown in Table S1, the total volume of the singleplex real-time PCR reaction was 20 μL, consisting of 10 μL of 2× AceQ qPCR Probe Master Mix (AceQ qPCR Probe Master Mix kit, Nanjing Vazyme biotechnology Co., Ltd.), 0.4 μL of each forward and reverse primer (10 μM), 0.2 μL of Taq Man probe (10 μM), 2 μL of template, and the remaining volume of nuclease-free water.

Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction, Concentration Assay

Journal: Virulence

Article Title: Development of a Taq Man-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses

doi: 10.1080/21505594.2020.1771980

Figure Lengend Snippet: Sensitivity of the multiplex real-time PCR assay.

Article Snippet: As shown in Table S1, the total volume of the singleplex real-time PCR reaction was 20 μL, consisting of 10 μL of 2× AceQ qPCR Probe Master Mix (AceQ qPCR Probe Master Mix kit, Nanjing Vazyme biotechnology Co., Ltd.), 0.4 μL of each forward and reverse primer (10 μM), 0.2 μL of Taq Man probe (10 μM), 2 μL of template, and the remaining volume of nuclease-free water.

Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction, Concentration Assay